|
KMID : 0352519930300030517
|
|
Korea Univercity Medical Journal
1993 Volume.30 No. 3 p.517 ~ p.527
|
|
|
Detection of HPV 6/11, 16/18 and 31/33/35 in CIN Ⅲ and Invasive Squamous Cell Carcinomas of the Uterine Cervix by Biotinylated In Situ Hybridization
|
|
|
|
|
Abstract
|
|
|
Human papillomavirus (HPV) is well known oncogenic virus implicated in the uterine cervical carcinomas. In this study, fourty-three cases of uterine cervix which were diagnosed as high grade CIN (22 cases) and invasive sqamous cell carcinomas (21 cases) without consideration of morphologic evidence of papillomavirus infection. In situ hybridization was done using MicroProbe Immunostaining System and biotinylated DNA probes 6/11, 16/18, and 31/33/35 from Live Technologies Inc.. in routinely processed formalin-fixed paraffin-embedded tissue sections.
The obtained results were summerized by the follows :
1. Twenty-three among 43 cases (53.5%) of high grade CIN and invasive squamous cell carcinomas were positive for HPV 16/18 and 31/33/35 DNA probes, however, none of the cases was positive for HPV 6/11 DNA probe.
2. Eleven cases of 22(50%) CIN Ill and 12 cases of 21(57.1%) invasive squamous cell carcinomas were positive for HPV 16//18 and 31/33/35 DNA probes, and 31/33/35 was more frequent than 16/18 subtype in invasive squamous cell carcinomas.
3. The HPV DNA 16/18 and 31/33/35 were detected in higher incidence in large cell nonkeratinizing squamous cell carcinoma than in keratinizing carcinoma.
4. The positive cells in intraepithelial neoplasia were more numerous in number than in invasive carcinomas. The positive reaction in the nuclei was homogeneous and strong in CIN, whereas it was granular and dot-like in invasive carcinomas.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|